The PNP Crime Laboratory traces its origin from te fingerprint Section of the G-2 Division of the Military Police Command Armed Forces in the West Pacific of thru United States Army (AFWESPAC), organized in May 19, 1945. Later on, it was upgarded into a branch and renamed as Crime Laboratory Branch of the Criminal Investigation Service(CIS), the investigative arm of the Philippine Constabulary (PC).

           The laboratory underwent several reorganizations and changes of names.   It was reoraganized on May 26, 1959 as a separate unit of the PC under table of Organization and Equipment 3-07(TO & E 3-07) of the Armed Forces of the Philippines and was renamed as PC Forensic Laboratory.    On December 1, 1960, pursuant to TO and E 03, the unit was renamed as the PC Central Crime Laboratory, and on June 28, 1961, it was activated as separate unit of the Armed Forces of the Philippines pursuant to Special Order Number 453 GHQ AFP.

           The National Headquarters of the Crime Laboratory in Camp Crame was constructed during the term of then Philippine Constabulary Chief, Brigadier General Vicente Raval on December 4, 1968.   The construction was completed on July 22, 1969, coinciding with its official desiganation as the PC Crime Laboratory(PCCL) pursuant to Headquarters Philippine Constabulary General Order Number 23.

           The Crime Laboraory saw contnuous improvement in the following years in order to serve the people more effectively.   In 1975, scientific equipment were acquired through the War Reparations Program of the Japanese Government.   Organizationally, the Crime Laboratory was expanded in 1978, under the authority of HPC GO Nr 23 wherein twelve(12) regional units were established.

           With the merging of the Philippine Constabulary and the Integrated National POlicxe (INP) on May 16, 1985, the PCCL was re-designated as PC/INP Crime Laboratory Service(CLS) as per HPC GO Nr 64.

           By virtue of Reublic Act 6975 (PNP Law) enacted in 1990, the PC/INP was abolished, paving the way for the creation of the Philippine National Police.  Consequently, the PNP Crime Laboratory Service was established as an Administrative Support Unit on January 30, 1991.  In 1996, the NAPOLCOM Resolution Nr 96-058 spelled out the strealining program of the PNP and designated the PNPCLS as a National Operational Support Unit.  Likewise, It dropped the word Sercie from the Crime Laboratory's name and would simply be known as the PNP Crime Laboratory.